Inputs needed for preparation:

1. Manual/initParams: directory storing the outputs of stalkInit (**.mrc/*.rec, *.mod, MOTL.csv, RotAxes.csv)
2. Recon/eTomo: directory storing reconstruction output (the folder where you perform eTomo reconstruction) (Optional)
3. Expand: the folder that stores the expanded results to be generated.

Output you will get:

Root directory

— Expand:

— tomograms_xxx.star

Below is the GUI used for generating the tomograms.star file.

• Initial Picking File Folder: Manual/initParams
• Tomo Reconstruction Folder: Recon/eTomo
• Output filename: Name your tomograms.star file. User customized
• Expand Folder: Expand result folder that defines the Expand output folder. User customized

GUI for generating tomograms.star file needed for expansion

You can modify the tomograms.star file based on your own setting. The file format follows the STAR convention.

data_

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