Inputs needed for preparation:

1. (Optional) A reference/template

Output you will get:

Root directory

— Expand:

— pick_xxx.params

Below is the GUI used for generating the pick.params file.

• Angular search
• Translational search
• Other Inputs

Note: The difference between the first round and the later round search range is that the first round takes the user-provided input, which is normally inaccurate for its center and angular orientation assignment, or the user only provides the particle center coordinates without angular info. Therefore, set the first round search range larger than the later round search. If no angular info is provided, the user needs to search the entire 180 degrees for the first round of angular search.

GUI for generating pick.params file needed for expansion

The output file will be stored in JSON format under the file name “pick.params.”